Phytochemicals from Mangifera pajang Kosterm and their biological activities

نویسندگان

  • Sadikah Ahmad
  • Mohd Aspollah Sukari
  • Nurussaadah Ismail
  • Intan Safinar Ismail
  • Ahmad Bustamam Abdul
  • Mohd Fadzelly Abu Bakar
  • Nurolaini Kifli
  • Gwendoline C L Ee
چکیده

BACKGROUND Mangifera pajang Kosterm is a plant species from the mango family (Anacardiaceae). The fruits are edible and have been reported to have high antioxidant content. However, the detailed phytochemical studies of the plant have not been reported previously. This study investigates the phytochemicals and biological activities of different parts of Mangifera pajang. METHODS The plant samples were extracted with solvents of different polarity to obtain the crude extracts. The isolated compounds were characterized using spectroscopic methods. The extracts and isolated compounds were subjected to cytotoxicity tests using human breast cancer (MCF-7), human cervical cancer (HeLa) and human colon cancer (HT-29) cells. The free radical scavenging activity test was conducted using the DPPH assay. Antimicrobial activity tests were carried out by using the disc diffusion method. RESULTS Phytochemical investigation on the kernel, stem bark and leaves of Mangifera pajang led to the isolation of methyl gallate (1), mixture of benzaldehyde (2) and benzyl alcohol (3), mangiferonic acid (4), 3β-hydroxy-cycloart-24-ene-26-oic acid (5), 3β,23-dihydroxy-cycloart-24-ene-26-oic acid (6), lupeol(7) lupenone(8), β-sitosterol(9), stigmasterol(10), trans-sobrerol(11) and quercitrin (12). Crude ethyl acetate and methanol extracts from the kernel indicated strong cytotoxic activity towards MCF-7 and HeLa cells with IC50 values of less than 10 μg/mL, while petroleum ether, chloroform and ethyl acetate extracts of the stem bark showed strong to moderate activity against MCF-7, HeLa and HT-29 cancer cell lines with IC50 values ranging from 5 to 30 μg/mL. As for the antimicrobial assays, only the ethyl acetate and methanol extracts from the kernel displayed some inhibition against the microbes in the antibacterial assays. The kernel extracts showed highest free radical scavenging activity with IC50 values of less than 10 μg/mL, while the ethyl acetate and methanol extracts of leaves displayed only weak activity in the DPPH assays. CONCLUSIONS Phytochemical investigations on various parts of Mangifera pajang have identified terpenoids and a flavonol derivative as major constituents. Bioassay studies have indicated that the crude extracts and isolated compounds have potential as naturally-derived anticancer and antimicrobial agents, besides possess high free radical scavenging activity.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Carotenoids from Mangifera Pajang and Their Antioxidant Capacity

This study provides new data on the various carotenoids found in bambangan (Mangifera pajang Kosterm.) peel and pulp extracts, such as all-trans-αand β-carotene, cis-β-carotene, 9-cis-β-carotene, and cryptoxanthin. Chemical and biological antioxidant assays were determined to evaluate the antioxidant capacity of bambangan peel and pulp extracts. Bambangan pulp had higher αand β-carotene content...

متن کامل

Determination of daidzein and genistein contents in mangifera fruit.

The aim of this study was to determine the daidzein and genistein contents in Mangifera fruits. Three Mangifera species namely 'bacang' (Mangifera foetida), 'kuini' (M. odorata) and 'bambangan' (M. pajang) each from two different locations were selected. The extraction of isoflavones was carried out at 80oC for 30, 60 and 90 min. HPLC method was performed with a flow rate of 1.00 ml/min using t...

متن کامل

Phytochemical Analysis of Leaf of Wedeliatrilobata, Achyranthesaspera and Chrysanthemum from the Twin–City (Durg & Bhilai), Chhattisgarh, India: A Comparative Study

The phytochemicals present in the plants are responsible for the biological activities and therapeutic properties of the various medicinal plants. They function as antioxidants in health promotion by preventing oxidant-damage which is the main cause of many diseases. The present study deals with the comparative analysis of phytochemicals in the methanolic and chloroformic leaf extracts of three...

متن کامل

Comparison Cytotoxic Effects of Mangifera Indica L. and Juglans Regia Aqueous Extract on Human Chronic Lymphocytic Leukemia

Natural products isolated from plant sources are well known for their pharmacological potential in diversity of disease treatments such as inflammatory or cancer conditions. Mango (Mangifera indica L.) and Juglans regia are thought to be rich of functional phytochemicals. To elucidate the anticancer activity of Juglans regia (JR) and Mangifera indica L (MI) aqueous extract were investigated on ...

متن کامل

Comparison Cytotoxic Effects of Mangifera Indica L. and Juglans Regia Aqueous Extract on Human Chronic Lymphocytic Leukemia

Natural products isolated from plant sources are well known for their pharmacological potential in diversity of disease treatments such as inflammatory or cancer conditions. Mango (Mangifera indica L.) and Juglans regia are thought to be rich of functional phytochemicals. To elucidate the anticancer activity of Juglans regia (JR) and Mangifera indica L (MI) aqueous extract were investigated on ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره 15  شماره 

صفحات  -

تاریخ انتشار 2015